5-Bromo-3-Indolyl-β-D-Galactopyranoside

(Bluo-Gal)
Inalco Code: 1758-0750
C.A.S.R.N.: 97753-82-7
Applications: Substrate for β-Galactosidase that is converted, by the enzyme, into an insoluble indigoblue chromophore, darker than the one released by X-Gal. In histological applications, Bluo-Gal produces a precipitate in the form of fine birefringent crystals which, upon microscopic examination under polarized light, emit a strong signal consisting of yellow reflected light, resulting in greatly enhanced sensitivity and better morphological resolution as compared with X-Gal. It is also used in Lac gene detection systems, in immunoblotting and immunocytochemical assays.
Formula: C14H16BrNO6

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